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Monitoring Enzyme Catalysis in the Multimeric State: Direct Observation of Arthrobacter 4-Hydroxybenzoyl-Coenzyme A Thioesterase Catalytic Complexes using Time-resolved Electrospray Ionization Mass Spectrometry

The ability to examine real time reaction kinetics for multimeric enzymes in their native state may offer unique insights toward understanding the catalytic mechanism and its interplay with three-dimensional structure. In this study, we have utilized a time-resolved electrospray mass spectrometry ap...

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Detalhes bibliográficos
Main Authors: Li, Zhili, Song, Feng, Zhuang, Zhihao, Dunaway-Mariano, Debra, Anderson, Karen S.
Formato: Artigo
Idioma:Inglês
Publicado em: 2009
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2743789/
https://ncbi.nlm.nih.gov/pubmed/19635449
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.ab.2009.07.030
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