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Preferential in vitro assembly of nucleosome cores on some AT-rich regions of SV40 DNA.

We have found that nucleosomes reconstituted from histone octamers and SV40 DNA Form I by progressively decreasing the salt concentration from 2 M NaCl are formed preferentially around 0.27, 0.37, 0.50 and 0.85 on SV40 DNA (relative to the EcoRI site). When SV40 DNA Form III is used, the nucleosomes...

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Bibliographic Details
Main Authors: Wasylyk, B, Oudet, P, Chambon, P
Format: Artigo
Language:Inglês
Published: 1979
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC328049/
https://ncbi.nlm.nih.gov/pubmed/228248
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