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Primer Extension Mutagenesis Powered by Selective Rolling Circle Amplification
Primer extension mutagenesis is a popular tool to create libraries for in vitro evolution experiments. Here we describe a further improvement of the method described by T.A. Kunkel using uracil-containing single-stranded DNA as the template for the primer extension by additional uracil-DNA glycosyla...
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| Main Authors: | , , , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Public Library of Science
2012
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3280210/ https://ncbi.nlm.nih.gov/pubmed/22355397 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0031817 |
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