Primer Extension Mutagenesis Powered by Selective Rolling Circle Amplification

Primer extension mutagenesis is a popular tool to create libraries for in vitro evolution experiments. Here we describe a further improvement of the method described by T.A. Kunkel using uracil-containing single-stranded DNA as the template for the primer extension by additional uracil-DNA glycosyla...

Popoln opis

Shranjeno v:
Bibliografske podrobnosti
Main Authors: Huovinen, Tuomas, Brockmann, Eeva-Christine, Akter, Sultana, Perez-Gamarra, Susan, Ylä-Pelto, Jani, Liu, Yuan, Lamminmäki, Urpo
Format: Artigo
Jezik:Inglês
Izdano: Public Library of Science 2012
Teme:
Research Article
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC3280210/
https://ncbi.nlm.nih.gov/pubmed/22355397
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0031817
Oznake: Označite
Brez oznak, prvi označite!

Podobne knjige/članki