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Cloning of soybean leghemoglobin structural gene sequences synthesized in vitro.
Double-stranded soybean leghemoglobin DNA was synthesized from leghemoglobin mRNA isolated from soybean nodules. The dsDNA was inserted into the Bam H1 site of plasmid pBR322 using the poly-dAT-joiner method. A cloned DNA fragment of one recombinant plasmid was isolated and characterized by restrict...
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| Hauptverfasser: | , , , , |
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| Format: | Artigo |
| Sprache: | Inglês |
| Veröffentlicht: |
1979
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| Online Zugang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC327917/ https://ncbi.nlm.nih.gov/pubmed/573890 |
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