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Cloning of soybean leghemoglobin structural gene sequences synthesized in vitro.

Double-stranded soybean leghemoglobin DNA was synthesized from leghemoglobin mRNA isolated from soybean nodules. The dsDNA was inserted into the Bam H1 site of plasmid pBR322 using the poly-dAT-joiner method. A cloned DNA fragment of one recombinant plasmid was isolated and characterized by restrict...

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Bibliographic Details
Main Authors: Truelsen, E, Gausing, K, Jochimsen, B, Jørgensen, P, Marcker, K A
Format: Artigo
Language:Inglês
Published: 1979
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC327917/
https://ncbi.nlm.nih.gov/pubmed/573890
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