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The use of R-looping for structural gene identification and mRNA purification.

A method is presented for the purification of mRNAs and the identification of structural gene sequences in recombinant DNA molecules. RNA is hybridized to double-stranded linear DNA such that R-loops are formed between most DNAs and their complementary RNA sequences. These R-loops are purified from...

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Bibliographic Details
Published in:Nucleic Acids Res
Main Authors: Woolford, J L, Rosbash, M
Format: Artigo
Language:Inglês
Published: Oxford University Press 1979
Online Access:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC327867/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/379820/
https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/6.7.2483
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