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Purification of specific adenovirus 2 RNAs by preparative hybridization and selective thermal elution.

A method is described for the preparation isolation of highly purified adenovirus RNA species. Cytoplasmic RNAs from cells infected with adenovirus 2 were selected by hybridization to viral DNA fragments bound to nitrocellulose membranes. A series of washes at elevated temperatures (50-70 degrees) d...

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Библиографические подробности
Опубликовано в::Nucleic Acids Res
Главные авторы: McGrogan, M, Spector, D J, Goldenberg, C J, Halbert, D, Raskas, H J
Формат: Artigo
Язык:Inglês
Опубликовано: Oxford University Press 1979
Online-ссылка:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC327715/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/424306/
https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/6.2.583
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