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Sequential Sepharose chromatographic isolation of polysomes and polysomal RNAs depleted in nuclear RNA from Xenopus.

Analyses of sequence complexities, stage specific gene expression, and mRNA sequence divergence require polysomal RNA preparations relatively free of nuclear RNA contamination. Conventional procedures for the isolation of uncontaminated polysomal RNAs which rely on sucrose density centrifugations ar...

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Autors principals: Swiderski, R E, Johnson, S A, Larkins, B A, Graham, D E
Format: Artigo
Idioma:Inglês
Publicat: 1979
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC327966/
https://ncbi.nlm.nih.gov/pubmed/493118
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