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Equilibria and kinetics of lac repressor-operator interactions by polyacrylamide gel electrophoresis.
We describe the use of gel electrophoresis in studies of equilibrium binding, site distribution, and kinetics of protein-DNA interactions. The method, which we call protein distribution analysis, is simple, sensitive and yields thermodynamically rigorous results. It is particularly well suited to st...
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| Publicado no: | Nucleic Acids Res |
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| Main Authors: | , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Oxford University Press
1981
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC327619/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/6275366/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/9.23.6505 |
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