Equilibria and kinetics of lac repressor-operator interactions by polyacrylamide gel electrophoresis.

We describe the use of gel electrophoresis in studies of equilibrium binding, site distribution, and kinetics of protein-DNA interactions. The method, which we call protein distribution analysis, is simple, sensitive and yields thermodynamically rigorous results. It is particularly well suited to st...

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Veröffentlicht in:	Nucleic Acids Res
Hauptverfasser:	Fried, M, Crothers, D M
Format:	Artigo
Sprache:	Inglês
Veröffentlicht:	Oxford University Press 1981
Schlagworte:	Research Article
Online-Zugang:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC327619/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/6275366/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/9.23.6505
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