Expression, purification, crystallization and preliminary crystallographic analysis of a thermostable DNA ligase from the archaeon Thermococcus sibiricus

DNA ligases join single-strand breaks in double-stranded DNA by catalyzing the formation of a phosphodiester bond between adjacent 5′-­phosphate and 3′-­hydroxyl termini. Their function is essential to maintain the integrity of the genome in DNA replication, recombination and repair. A recombinant A...

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Main Authors: Petrova, T. E., Bezsudnova, E. Y., Dorokhov, B. D., Slutskaya, E. S., Polyakov, K. M., Dorovatovskiy, P. V., Ravin, N. V., Skryabin, K. G., Kovalchuk, M. V., Popov, V. O.
格式: Artigo
語言:Inglês
出版: International Union of Crystallography 2012
主題:
Crystallization Communications
在線閱讀:https://ncbi.nlm.nih.gov/pmc/articles/PMC3274393/
https://ncbi.nlm.nih.gov/pubmed/22297989
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S1744309111050913
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