Overexpression, purification and crystallization of a thermostable DNA ligase from the archaeon Thermococcus sp. 1519

DNA ligases catalyze the sealing of 5′-phosphate and 3′-hydroxyl termini at single-strand breaks in double-stranded DNA and their function is essential to maintain the integrity of the genome in DNA metabolism. An ATP-dependent DNA ligase from the archaeon Thermococcus sp. 1519 was overexpressed, pu...

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Bibliografski detalji
Glavni autori: Bezsudnova, E. Y., Kovalchuk, M. V., Mardanov, A. V., Poliakov, K. M., Popov, V. O., Ravin, N. V., Skryabin, K. G., Smagin, V. A., Stekhanova, T. N., Tikhonova, T. V.
Format: Artigo
Jezik:Inglês
Izdano: International Union of Crystallography 2009
Teme:
Crystallization Communications
Online pristup:https://ncbi.nlm.nih.gov/pmc/articles/PMC2664762/
https://ncbi.nlm.nih.gov/pubmed/19342782
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S1744309109007799
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