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Error correction of microchip synthesized genes using Surveyor nuclease

The development of economical and high-throughput gene synthesis technology has been hampered by the high occurrence of errors in the synthesized products, which requires expensive labor and time to correct. Here, we describe an error correction reaction (ECR), which employs Surveyor, a mismatch-spe...

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Detalhes bibliográficos
Main Authors: Saaem, Ishtiaq, Ma, Siying, Quan, Jiayuan, Tian, Jingdong
Formato: Artigo
Idioma:Inglês
Publicado em: Oxford University Press 2012
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3273826/
https://ncbi.nlm.nih.gov/pubmed/22127863
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkr887
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