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A versatile element for gene addition in bacterial chromosomes

The increasing interest in genetic manipulation of bacterial host metabolic pathways for protein or small molecule production has led to a need to add new genes to a chromosome quickly and easily without leaving behind a selectable marker. The present report describes a vector and four-day procedure...

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Detalles Bibliográficos
Main Authors: Sibley, Marion H., Raleigh, Elisabeth A.
Formato: Artigo
Idioma:Inglês
Publicado: Oxford University Press 2012
Assuntos:
Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC3273789/
https://ncbi.nlm.nih.gov/pubmed/22123741
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkr1085
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