Shotgun DNA sequencing using cloned DNase I-generated fragments.
A method for DNA sequencing has been developed that utilises libraries of cloned randomly-fragmented DNA. The DNA to be sequenced is first subjected to limit attach by a non-specific endonuclease (DNase I in the presence of Mn++), fractionated by size and cloned in a single-stranded phage vector. Cl...
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| Pubblicato in: | Nucleic Acids Res |
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| Autore principale: | |
| Natura: | Artigo |
| Lingua: | Inglês |
| Pubblicazione: |
Oxford University Press
1981
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| Accesso online: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC327328/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/6269069/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/9.13.3015 |
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