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Shotgun DNA sequencing using cloned DNase I-generated fragments.
A method for DNA sequencing has been developed that utilises libraries of cloned randomly-fragmented DNA. The DNA to be sequenced is first subjected to limit attach by a non-specific endonuclease (DNase I in the presence of Mn++), fractionated by size and cloned in a single-stranded phage vector. Cl...
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| Príomhúdar: | |
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| Formáid: | Artigo |
| Teanga: | Inglês |
| Foilsithe: |
1981
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| Ábhair: | |
| Rochtain Ar Líne: | https://ncbi.nlm.nih.gov/pmc/articles/PMC327328/ https://ncbi.nlm.nih.gov/pubmed/6269069 |
| Clibeanna: |
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