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Nonlinear structured-illumination microscopy with a photoswitchable protein reveals cellular structures at 50-nm resolution

Using ultralow light intensities that are well suited for investigating biological samples, we demonstrate whole-cell superresolution imaging by nonlinear structured-illumination microscopy. Structured-illumination microscopy can increase the spatial resolution of a wide-field light microscope by a...

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Detalhes bibliográficos
Main Authors: Rego, E. Hesper, Shao, Lin, Macklin, John J., Winoto, Lukman, Johansson, Göran A., Kamps-Hughes, Nicholas, Davidson, Michael W., Gustafsson, Mats G. L.
Formato: Artigo
Idioma:Inglês
Publicado em: National Academy of Sciences 2012
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3271870/
https://ncbi.nlm.nih.gov/pubmed/22160683
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.1107547108
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