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Super-Resolution Video Microscopy of Live Cells by Structured Illumination

Structured-illumination microscopy can double the resolution of the wide-field fluorescence microscope, but has previously been too slow for dynamic live imaging. Here we demonstrate a high-speed SIM that is capable of 100 nm resolution at frame rates up to 11 Hz for several hundred time frames. We...

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Detalhes bibliográficos
Main Authors: Kner, Peter, Chhun, Bryant B., Griffis, Eric R., Winoto, Lukman, Gustafsson, Mats G. L.
Formato: Artigo
Idioma:Inglês
Publicado em: 2009
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2895555/
https://ncbi.nlm.nih.gov/pubmed/19404253
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nmeth.1324
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