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Monodisperse, “highly” positively charged protein polymer drag-tags generated in an intein-mediated purification system used in free-solution electrophoretic separations of DNA

Free-solution conjugate electrophoresis (FSCE) is a method of DNA sequencing that eliminates the need for viscous polymer solutions by tethering a carefully designed, mobility modifying “drag-tag” to each DNA molecule to achieve size-based separations of DNA. The most successful drag-tags to date ar...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser: Wang, Xiaoxiao, Albrecht, Jennifer Coyne, Lin, Jennifer S., Barron, Annelise E.
Format: Artigo
Sprache:Inglês
Veröffentlicht: 2011
Schlagworte:
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC3270947/
https://ncbi.nlm.nih.gov/pubmed/22168388
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/bm2013313
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