Procedure for C2 deuteration of nucleic acids and determination of A psi 31 pseudouridine conformation by nuclear Overhauser effect in yeast tRNAPhe.

Nuclear Overhauser effect (NOE) combined with semispecific deuteration provides a general strategy for identification of exchangeable protons in nucleic base pairs, and has been extended to NOEs involving purine C2 protons in tRNA. Deuterated tri-ethyl orthoformate was condensed with 5(4)-amino imid...

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Bibliografiske detaljer
Udgivet i:Nucleic Acids Res
Main Authors: Roy, S, Papastavros, M Z, Redfield, A G
Format: Artigo
Sprog:Inglês
Udgivet: Oxford University Press 1982
Online adgang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC327090/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/6761652/
https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/10.24.8341
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