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Procedure for C2 deuteration of nucleic acids and determination of A psi 31 pseudouridine conformation by nuclear Overhauser effect in yeast tRNAPhe.

Nuclear Overhauser effect (NOE) combined with semispecific deuteration provides a general strategy for identification of exchangeable protons in nucleic base pairs, and has been extended to NOEs involving purine C2 protons in tRNA. Deuterated tri-ethyl orthoformate was condensed with 5(4)-amino imid...

Deskribapen osoa

Gorde:
Xehetasun bibliografikoak
Egile Nagusiak: Roy, S, Papastavros, M Z, Redfield, A G
Formatua: Artigo
Hizkuntza:Inglês
Argitaratua: 1982
Sarrera elektronikoa:https://ncbi.nlm.nih.gov/pmc/articles/PMC327090/
https://ncbi.nlm.nih.gov/pubmed/6761652
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