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Physical parameters affecting the rate and completion of RNA driven hybridization of DNA: new measurements relevant to quantitation based on kinetics.

Differences in the RNA-driven hybridization kinetics of genomic DNA and cDNA probes led us to examine physical parameters affecting these reactions. Cloned cDNA complementary to serum albumin (SA) mRNA hybridized in accordance with single component kinetics, whereas cloned SA genomic DNA hybridized...

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Detalhes bibliográficos
Main Authors: Van Ness, J, Hahn, W E
Formato: Artigo
Idioma:Inglês
Publicado em: 1982
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC327070/
https://ncbi.nlm.nih.gov/pubmed/6186985
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