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Capillary electrophoresis-laser induced fluorescence (CE-LIF) assay for measurement of intra-cellular D-Serine and serine racemase activity

An enantioselective capillary electrophoresis–laser induced fluorescence (CE-LIF) method for the analysis of D-Serine (D-Ser) in cellular matrices has been developed. The assay involves derivatization with FITC followed by CE-LIF using 0.5 mM hydroxyl propyl-β-cyclodextrin in borate buffer [80 mM, p...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser: Singh, Nagendra S., Paul, Rajib K., Sichler, Megan, Moaddel, Ruin, Bernier, Michel, Wainer, Irving W.
Format: Artigo
Sprache:Inglês
Veröffentlicht: 2011
Schlagworte:
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC3270116/
https://ncbi.nlm.nih.gov/pubmed/22037294
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.ab.2011.10.003
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