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Determination of free and protein-bound glutathione in HepG2 cells using capillary electrophoresis with laser-induced fluorescence detection

A rapid method using capillary electrophoresis with laser-induced fluorescence detection (CE-LIF) was developed to determine free and protein-bound glutathione (GSH) in human HepG2 hepatocarcinoma cells. The samples were derivatized with 5-iodoacetamidofluorescein (5-IAF), and analyzed at 22 kV usin...

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Detalhes bibliográficos
Main Authors: Wang, Yan, Xie, Yi, Bernier, Michel, Wainer, Irving W.
Formato: Artigo
Idioma:Inglês
Publicado em: 2008
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2663004/
https://ncbi.nlm.nih.gov/pubmed/18602637
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.chroma.2008.06.027
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