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Functional assay of tRNA molecules transcribed from a purified gene.
Purified tRNA genes are expressed when microinjected into the nucleus of X.laevis oocytes. In this paper we describe a method to assay the capacity to be aminoacylated of the tRNA transcribed in the frog oocytes. The method exploits the radiochemical purity of the transcript and relies on the bindin...
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| Main Authors: | , , , , , , |
|---|---|
| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
1982
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| Online adgang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC327009/ https://ncbi.nlm.nih.gov/pubmed/6891456 |
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