Functional assay of tRNA molecules transcribed from a purified gene.

Purified tRNA genes are expressed when microinjected into the nucleus of X.laevis oocytes. In this paper we describe a method to assay the capacity to be aminoacylated of the tRNA transcribed in the frog oocytes. The method exploits the radiochemical purity of the transcript and relies on the bindin...

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Bibliografische Detailangaben

Veröffentlicht in:	Nucleic Acids Res
Hauptverfasser:	Corbo, L, Ciliberto, G, Traboni, C, Santamaria, R, Cimino, F, Cortese, R, Salvatore, F
Format:	Artigo
Sprache:	Inglês
Veröffentlicht:	Oxford University Press 1982
Online-Zugang:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC327009/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/6891456/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/10.22.7363
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