Functional assay of tRNA molecules transcribed from a purified gene.
Purified tRNA genes are expressed when microinjected into the nucleus of X.laevis oocytes. In this paper we describe a method to assay the capacity to be aminoacylated of the tRNA transcribed in the frog oocytes. The method exploits the radiochemical purity of the transcript and relies on the bindin...
Spremljeno u:
| Izdano u: | Nucleic Acids Res |
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| Glavni autori: | , , , , , , |
| Format: | Artigo |
| Jezik: | Inglês |
| Izdano: |
Oxford University Press
1982
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| Online pristup: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC327009/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/6891456/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/10.22.7363 |
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