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An Orthogonal Purification Strategy for Isolating Crosslinked Domains of Modular Synthases
Chemo-enzymatic methods for covalently crosslinking carrier proteins with partner enzymes within modular synthases holds promise for elucidating and engineering metabolic pathways. Our efforts to crystallize the ACP-KS complexes of fatty acid synthases have been complicated by difficulties in the pu...
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| Main Authors: | , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2008
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3269958/ https://ncbi.nlm.nih.gov/pubmed/18249538 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.bmcl.2008.01.026 |
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