Improvement of Thermal Stability via Outer-Loop Ion Pair Interaction of Mutated T1 Lipase from Geobacillus zalihae Strain T1

Mutant D311E and K344R were constructed using site-directed mutagenesis to introduce an additional ion pair at the inter-loop and the intra-loop, respectively, to determine the effect of ion pairs on the stability of T1 lipase isolated from Geobacillus zalihae. A series of purification steps was app...

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Hlavní autoři: Ruslan, Rudzanna, Abd. Rahman, Raja Noor Zaliha Raja, Leow, Thean Chor, Ali, Mohd Shukuri Mohamad, Basri, Mahiran, Salleh, Abu Bakar
Médium: Artigo
Jazyk:Inglês
Vydáno: Molecular Diversity Preservation International (MDPI) 2012
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On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC3269730/
https://ncbi.nlm.nih.gov/pubmed/22312296
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3390/ijms13010943
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