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The construction of a synthetic Escherichia coli trp promoter and its use in the expression of a synthetic interferon gene.

An 82 base pair DNA fragment has been synthesised which contains the E. coli trp promoter and operator sequences and also encodes the first Shine Dalgarno sequence of the trp operon. This DNA fragment is flanked by EcoRI and ClaI/TaqI cohesive ends and is thus easy to clone, transfer between vector...

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Detalhes bibliográficos
Main Authors: Windass, J D, Newton, C R, De Maeyer-Guignard, J, Moore, V E, Markham, A F, Edge, M D
Formato: Artigo
Idioma:Inglês
Publicado em: 1982
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC326954/
https://ncbi.nlm.nih.gov/pubmed/6184675
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