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Bacteriophage T7 late promoters: construction and in vitro transcription properties of deletion mutants.

The construction of plasmids containing T7 class I promoters with deletion mutants was described. Restriction fragments, ending at the Hinf I site located at position -10 in the promoter from 14.8% of the T7 genome, were cloned into pBR322. This produced the deletion of either the left or the right...

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Bibliographische Detailangaben
Veröffentlicht in:Nucleic Acids Res
Hauptverfasser: Chapman, K A, Wells, R D
Format: Artigo
Sprache:Inglês
Veröffentlicht: Oxford University Press 1982
Online Zugang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC326922/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/6294599/
https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/10.20.6331
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