Sequence specific cleavage of DNA by micrococcal nuclease.
Micrococcal nuclease is shown to cleave DNA under conditions of partial digestion in a specific manner. Sequences of the type 5'CATA and 5'CTA are attacked preferentially, followed by exonucleolytic degradation at the newly generated DNA termini. GC-rich flanking sequences further increase the proba...
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| Veröffentlicht in: | Nucleic Acids Res |
|---|---|
| Hauptverfasser: | , |
| Format: | Artigo |
| Sprache: | Inglês |
| Veröffentlicht: |
Oxford University Press
1981
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| Online-Zugang: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC326882/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/7279658/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/9.12.2643 |
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