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Simple Preparation of Pacific Cod Trypsin for Enzymatic Peptide Synthesis
Trypsin from the pyloric caeca of Pacific cod (Gadus macrocephalus) was easily prepared by affinity chromatography on Benzamidine Sepharose 6B and gel filtration on Superdex 75. Pacific cod trypsin was composed of three isozymes, and their molecular masses were estimated 23,756.34 Da, 23,939.62 Da,...
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| Main Authors: | , , , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
SAGE-Hindawi Access to Research
2011
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3268023/ https://ncbi.nlm.nih.gov/pubmed/22312475 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.4061/2011/912382 |
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