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In Vitro Assembly of Multiple DNA Fragments Using Successive Hybridization
Construction of recombinant DNA from multiple fragments is widely required in molecular biology, especially for synthetic biology purposes. Here we describe a new method, successive hybridization assembling (SHA) which can rapidly do this in a single reaction in vitro. In SHA, DNA fragments are prep...
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| Main Authors: | , , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Public Library of Science
2012
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3266897/ https://ncbi.nlm.nih.gov/pubmed/22291927 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0030267 |
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