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Multiplex Real-Time PCR Assay and Melting Curve Analysis for Identifying Mycobacterium tuberculosis Complex and Nontuberculous Mycobacteria

A multiplex real-time PCR assay and melting curve analysis for identifying 23 mycobacterial species was developed and evaluated using 77 reference strains and 369 clinical isolates. Concordant results were obtained for all 189 (100%) isolates of the Mycobacterium tuberculosis complex and 169 (93.9%)...

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Autori principali: Kim, Jeong-Uk, Cha, Choong-Hwan, An, Hae-Kyong
Natura: Artigo
Lingua:Inglês
Pubblicazione: American Society for Microbiology 2012
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Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC3264165/
https://ncbi.nlm.nih.gov/pubmed/22162553
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JCM.06155-11
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