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Identification and amplification of the E. coli phr gene product.
We have constructed a series of multicopy plasmids that complement mutations in the phr gene of Escherichia coli. By subcloning into a tac plasmid vector we obtained a phr plasmid that upon induction overproduces two proteins of Mr's 49,000 and 20,000. Tn1000 insertions into the phr gene caused...
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| Главные авторы: | , , |
|---|---|
| Формат: | Artigo |
| Язык: | Inglês |
| Опубликовано: |
1983
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| Online-ссылка: | https://ncbi.nlm.nih.gov/pmc/articles/PMC326406/ https://ncbi.nlm.nih.gov/pubmed/6314252 |
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