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Identification and amplification of the E. coli phr gene product.
We have constructed a series of multicopy plasmids that complement mutations in the phr gene of Escherichia coli. By subcloning into a tac plasmid vector we obtained a phr plasmid that upon induction overproduces two proteins of Mr's 49,000 and 20,000. Tn1000 insertions into the phr gene caused...
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| 出版年: | Nucleic Acids Res |
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| 主要な著者: | , , |
| フォーマット: | Artigo |
| 言語: | Inglês |
| 出版事項: |
Oxford University Press
1983
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| オンライン･アクセス: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC326406/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/6314252/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/11.19.6667 |
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