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Efficient site-directed mutagenesis by simultaneous use of two primers.
A rapid and efficient procedure for site specific mutagenesis is described. A double primed synthesis with a 17-mer mismatch primer and a "universal" 15-mer M13 sequencing primer was used to introduce a T to A transversion into an ompF signal peptide gene cloned in the M13mp8 vector. The t...
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| Hlavní autoři: | , , , |
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| Médium: | Artigo |
| Jazyk: | Inglês |
| Vydáno: |
1983
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| On-line přístup: | https://ncbi.nlm.nih.gov/pmc/articles/PMC326240/ https://ncbi.nlm.nih.gov/pubmed/6308572 |
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