Efficient site-directed mutagenesis by simultaneous use of two primers.
A rapid and efficient procedure for site specific mutagenesis is described. A double primed synthesis with a 17-mer mismatch primer and a "universal" 15-mer M13 sequencing primer was used to introduce a T to A transversion into an ompF signal peptide gene cloned in the M13mp8 vector. The two primers...
Enregistré dans:
| Publié dans: | Nucleic Acids Res |
|---|---|
| Auteurs principaux: | , , , |
| Format: | Artigo |
| Langue: | Inglês |
| Publié: |
Oxford University Press
1983
|
| Accès en ligne: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC326240/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/6308572/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/11.15.5103 |
| Tags: |
Pas de tags, Soyez le premier à ajouter un tag!
|