Single/low-copy integration of transgenes in Caenorhabditis elegans using an ultraviolet trimethylpsoralen method

BACKGROUND: Transgenic strains of Caenorhabditis elegans are typically generated by injecting DNA into the germline to form multi-copy extrachromosomal arrays. These transgenes are semi-stable and their expression is silenced in the germline. Mos1 transposon or microparticle bombardment methods have...

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Главные авторы: Kage-Nakadai, Eriko, Kobuna, Hiroyuki, Funatsu, Osamu, Otori, Muneyoshi, Gengyo-Ando, Keiko, Yoshina, Sawako, Hori, Sayaka, Mitani, Shohei
Формат: Artigo
Язык:Inglês
Опубликовано: BioMed Central 2012
Предметы:
Methodology Article
Online-ссылка:https://ncbi.nlm.nih.gov/pmc/articles/PMC3262153/
https://ncbi.nlm.nih.gov/pubmed/22217006
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1472-6750-12-1
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