Oligonucleotide-directed mutagenesis of gene IX of bacteriophage M13.
The synthetic oligodeoxyribonucleotide pCGAAAGACTACAC has been applied as a site-specific mutagen to introduce a T leads to G transversion mutation at nucleotide position 1223 of the M13 DNA sequence. The in vitro-induced conversion of a TAT codon into a TAG at this position resulted in gene IX muta...
Uloženo v:
| Vydáno v: | Nucleic Acids Res |
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| Hlavní autoři: | , , , , |
| Médium: | Artigo |
| Jazyk: | Inglês |
| Vydáno: |
Oxford University Press
1982
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| Témata: | |
| On-line přístup: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC326205/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/6278437/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/10.3.821 |
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