Oligonucleotide-directed mutagenesis of gene IX of bacteriophage M13.

The synthetic oligodeoxyribonucleotide pCGAAAGACTACAC has been applied as a site-specific mutagen to introduce a T leads to G transversion mutation at nucleotide position 1223 of the M13 DNA sequence. The in vitro-induced conversion of a TAT codon into a TAG at this position resulted in gene IX muta...

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Main Authors: Simons, G F, Veeneman, G H, Konings, R N, van Boom, J H, Schoemakers, J G
Format: Artigo
Jezik:Inglês
Izdano: 1982
Teme:
Research Article
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC326205/
https://ncbi.nlm.nih.gov/pubmed/6278437
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