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Chemical crosslinking of elongation factor G to the 23S RNA in 70S ribosomes from Escherichia coli.
Elongation factor G was crosslinked to the 23S RNA of 70S Escherichia coli ribosomes with the bifunctional, cleavable reagent diepoxybutane (DEB). The EF-G-23S RNA complex was isolated and digested with ribonuclease A. After digestion, an RNA fragment, protected by EF-G was cleaved from the complex...
保存先:
| 出版年: | Nucleic Acids Res |
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| 第一著者: | |
| フォーマット: | Artigo |
| 言語: | Inglês |
| 出版事項: |
Oxford University Press
1983
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| オンライン･アクセス: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC326095/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/6348702/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/11.14.4923 |
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