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Chemical crosslinking of elongation factor G to the 23S RNA in 70S ribosomes from Escherichia coli.
Elongation factor G was crosslinked to the 23S RNA of 70S Escherichia coli ribosomes with the bifunctional, cleavable reagent diepoxybutane (DEB). The EF-G-23S RNA complex was isolated and digested with ribonuclease A. After digestion, an RNA fragment, protected by EF-G was cleaved from the complex...
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| Hovedforfatter: | |
|---|---|
| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
1983
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| Online adgang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC326095/ https://ncbi.nlm.nih.gov/pubmed/6348702 |
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