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Cloning exons of mapping of transcription: characterization of the Drosophila melanogaster alcohol dehydrogenase gene.

A novel method has been developed for determining the location of RNA termini and intron-exon boundaries using genomic clones. RNA is hybridized to a single-stranded M13 phage derivative of the genomic fragment of interest. S1 nuclease digestion results in an RNA-DNA hybrid corresponding to any tran...

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Detalhes bibliográficos
Autor principal: Henikoff, S
Formato: Artigo
Idioma:Inglês
Publicado em: 1983
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC326083/
https://ncbi.nlm.nih.gov/pubmed/6410356
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