Cloning exons of mapping of transcription: characterization of the Drosophila melanogaster alcohol dehydrogenase gene.
A novel method has been developed for determining the location of RNA termini and intron-exon boundaries using genomic clones. RNA is hybridized to a single-stranded M13 phage derivative of the genomic fragment of interest. S1 nuclease digestion results in an RNA-DNA hybrid corresponding to any tran...
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| Publicado no: | Nucleic Acids Res |
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| Autor principal: | |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Oxford University Press
1983
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| Acesso em linha: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC326083/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/6410356/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/11.14.4735 |
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