Extensively Hydrated but Folded: A Novel State of Globular Proteins Stabilized at High Pressure and Low Temperature

We studied conformational fluctuations of the transcription factor c-Myb R2 subdomain (52 residues with three Trp) at high pressure and low temperature (5°C) using two different spectroscopic methods, Trp fluorescence and (1)H NMR, on its chemically stable mutant C130I (pseudo-wild-type (WT(S))), wh...

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Detalhes bibliográficos
Main Authors: Puthenpurackal Narayanan, Sunilkumar, Maeno, Akihiro, Matsuo, Hiroshi, Oda, Masayuki, Morii, Hisayuki, Akasaka, Kazuyuki
Formato: Artigo
Idioma:Inglês
Publicado em: The Biophysical Society 2012
Assuntos:
Biophysical Letter
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3260745/
https://ncbi.nlm.nih.gov/pubmed/22339877
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.bpj.2011.12.015
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