Effect of the C-terminal Truncation on the Functional Cycle of Chaperonin GroEL: IMPLICATION THAT THE C-TERMINAL REGION FACILITATES THE TRANSITION FROM THE FOLDING-ARRESTED TO THE FOLDING-COMPETENT STATE

To elucidate the exact role of the C-terminal region of GroEL in its functional cycle, the C-terminal 20-amino acid truncated mutant of GroEL was constructed. The steady-state ATPase rate and duration of GroES binding showed that the functional cycle of the truncated GroEL is extended by ∼2 s in com...

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Detalhes bibliográficos
Main Authors: Suzuki, Mihoko, Ueno, Taro, Iizuka, Ryo, Miura, Takahiro, Zako, Tamotsu, Akahori, Rena, Miyake, Takeo, Shimamoto, Naonobu, Aoki, Mutsuko, Tanii, Takashi, Ohdomari, Iwao, Funatsu, Takashi
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Biochemistry and Molecular Biology 2008
Assuntos:
Protein Structure and Folding
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3259756/
https://ncbi.nlm.nih.gov/pubmed/18583344
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1074/jbc.M804090200
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