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Further improvements on the phosphotriester synthesis of deoxyribooligonucleotides and the oligonucleotide directed site-specific mutagenesis of E. coli lipoprotein gene.

Two improvements that greatly enhance the rate of phosphotriester oligonucleotide synthesis are described: 1) use of hindered primary amines, e.g. t-butyl amine for decyanoethylation of oligonucleotide triester intermediates, and 2) a simplified isolation procedure that eliminates the tedious bicarb...

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Detalles Bibliográficos
Autores principales: Hsiung, H, Inouye, S, West, J, Sturm, B, Inouye, M
Formato: Artigo
Lenguaje:Inglês
Publicado: 1983
Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC325959/
https://ncbi.nlm.nih.gov/pubmed/6344008
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