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Cloning and expression of the Staphylococcus aureus protein A gene in Escherichia coli.

A gene bank of Staphylococcus aureus strain Cowan I was established using an E. coli HB101/pBR327 host-vector system. Recombinants expressing staphylococcal protein A (SPA) were detected using an IgG-binding assay. A 3.2 Kb DNA fragment directing the synthesis of SPA in E. coli was identified. SPA p...

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Main Authors: Duggleby, C J, Jones, S A
Formato: Artigo
Idioma:Inglês
Publicado: 1983
Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC325948/
https://ncbi.nlm.nih.gov/pubmed/6304620
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