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Cloning and expression of the Staphylococcus aureus protein A gene in Escherichia coli.
A gene bank of Staphylococcus aureus strain Cowan I was established using an E. coli HB101/pBR327 host-vector system. Recombinants expressing staphylococcal protein A (SPA) were detected using an IgG-binding assay. A 3.2 Kb DNA fragment directing the synthesis of SPA in E. coli was identified. SPA p...
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| Hauptverfasser: | , |
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| Format: | Artigo |
| Sprache: | Inglês |
| Veröffentlicht: |
1983
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| Online Zugang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC325948/ https://ncbi.nlm.nih.gov/pubmed/6304620 |
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